Part:BBa_K4467003

PstHXT1 (from Puccinia striiformis var. striiformis)

Profile

Gene Name: PstHXT1
Subcellular localisation: Plasma membrane
Length (bp): 1596
Amino acids length: 530
Organism of origin: Puccinia striiformis var. striiformis

Properties and Biology

PstHXT1 is a hexose transporter protein of 56.8kDa molecular weight and is predicted to contain 12 transmembrane domains (Chang et. al., 2020). Localised exclusively to the plasma membrane of the haustoriun structure, PstHXT1 is thought to be integral in establishing an interaction between wheat and Puccinia rust species (Garnica et. al., 2014). Experimentally, this protein has been successfully expressed in the yeast mutant strain EBY.VW4000 (Chang et. al., 2020).

Usage

As part of the 2022 UNSW_Australia project to design a peptide based inhibitor for Puccinia sp., we used PstHXT1 as a potential target. This protein was expressed in NEB TURBO E.coli cells.

References

Chang, Q. et al. (2020) "Hexose transporter Ps HXT1‐mediated sugar uptake is required for pathogenicity of wheat stripe rust", Plant Biotechnology Journal, 18(12), pp. 2367-2369. doi: 10.1111/pbi.13398. Garnica, D. et al. (2014) "The Ins and Outs of Rust Haustoria", PLoS Pathogens, 10(9), p. e1004329. doi: 10.1371/journal.ppat.1004329.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 878
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 715
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 573
  Illegal BsaI.rc site found at 1494